Carnation etched ring virus (CERV) is a plant pararetrovirus species of the genus Caulimovirus (Caulimoviridae family, International Committee on the Taxonomy of Viruses -ICTV- Fauquet et al. 2005) that causes the "Etched ring disease" on carnation plants (Dianthus caryophyllus) characterized by necrotic flecks, rings and line patterns on the leaves. The CERV Indian isolate has been included in our phylogenetic analyses (see, Llorens et al 2009).

CERV isometric virions are composed of a genomic circular double-stranded DNA (7924 bp long) encoding for six Open reading frames (ORFs) whose size and organization are similar to those of known caulimoviruses (Raikhy et al. 2005, unpublished; Hull et al. 1986). The aminoacids sequences of the protein products show similarities with those encoded by the corresponding ORFs of known Caulimoviruses. ORF I encodes the movement protein (MOV), ORF II and ORF III are similar to the "aphid transmission factor" (ATF) and "virion associated protein" (VAP), respectively. ORF IV encodes the COAT protein and contains the "RNA-binding" motif characteristic of the Caulimoviridae Gag-like domain (C-X-C-X2-C-X4-H-X4-C ) (Bouhida et al. 1993;Hull 1996; Llorens et al 2009). ORF V product is a polyprotein displaying the typical Pol domains -aspartic protease (PR), reverse transcriptase (RT) and RNase H (RH) – and finally ORF VI encodes for a "Translational transactivator protein" (TAV) (Raikhy et al. 2005, unpublished). Between ORFs V (Pol) and VI (TAV) the virus presents a short intergenic region like those observed in the genome of other Caulimovirus species such as EVCV and LLDAV. A second larger intergenic region is downstream to the ORF VI. A promoter, located in the 3′ terminus of ORF VI and extending into the large intergenic region of CERV (Kuluev and Chemeris 2007), has been demonstrated to display an expression activity similar or more efficient in some plants than that of the CaMV 35S promoter (Kuluev et al. 2008).



Figure not to scale. If present, long terminal repeats (LTRs) have been highlighted in blue. Amino acid motifs noted with lines indicate the conserved residues in each protein domain, abbreviations below mean:

MA=matrix PR=protease DU or DUT=dUTPase TM=transmembrane TAV or IBMP=transactivator/viroplasmin or inclusion body matrix protein
CA=capsid RT=reverse transcriptase INT=Integrase CHR=chromodomain
NC=nucleocapsid RH=RNaseH SU=surface MOV=movement protein
PPT=polypurine tract PBS=primer binding site ATF=aphid transmission factor VAP=virion associated protein

Related literature

Genbank accession: 55250904
Clade: '
Cluster or genus: Caulimovirus
Branch or class: Class 1
Family: Caulimoviridae
System: LTR retroelements
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Dianthus caryophyllus.jpg
Dianthus caryophyllus
Wikipedia, courtesy of "KeepOpera"
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Welcome to the Gypsy Database (GyDB) an open editable database about the evolutionary relationship of viruses, mobile genetic elements (MGEs) and the genomic repeats where we invite all authors to contribute with their knowledge to improve and expand the topics.
Cite this project:

Llorens, C., Futami, R., Covelli, L., Dominguez-Escriba, L., Viu, J.M., Tamarit, D., Aguilar-Rodriguez, J. Vicente-Ripolles, M., Fuster, G., Bernet, G.P., Maumus, F., Munoz-Pomer, A., Sempere, J.M., LaTorre, A., Moya, A. (2011) The Gypsy Database (GyDB) of Mobile Genetic Elements: Release 2.0 Nucleic Acids Research (NARESE) 39 (suppl 1): D70-D74 doi: 10.1093/nar/gkq1061

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