DescriptionGrande is a LTR retroelement originally characterized in the genome of Zea diploperennis, and known to also be widely distributed in all Mais chromosomes (Aledo et al. 1995). This element belongs to a lineage of plant LTR retrotransposons (Tat), sibling of other lineage of putative plant endogenous retroviruses (Athila), providing evidence of a shared ancestral lineage originally described as class A by Wright and Voytas (1998). The genomic structure of Grande has several subtypes, subtype 1-4 is taken as representative. The genome structure of this element is 13.8 Kb in size including LTRs of 0.6 Kb. The internal region displays a Primer Binding Site (PBS) complementary to a tRNA Arg, three Open Reading Frames (ORFs) for gag, pro, and pol genes, and downstream to the integrase domain, a non-coding region of approximately 7 Kb in size, which contains two tandem repeats and an ORF that might be transcriptionally active in reverse frame, and also, a Polypurine Tract (PPT) adjacent to the 3´LTR (Martínez-Izquierdo, García-Martínez and Vicient, 1997; García-Martínez and Martínez-Izquierdo 2003). Structure
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Llorens, C., Futami, R., Covelli, L., Dominguez-Escriba, L., Viu, J.M., Tamarit, D., Aguilar-Rodriguez, J. Vicente-Ripolles, M., Fuster, G., Bernet, G.P., Maumus, F., Munoz-Pomer, A., Sempere, J.M., LaTorre, A., Moya, A. (2011) The Gypsy Database (GyDB) of Mobile Genetic Elements: Release 2.0 Nucleic Acids Research (NARESE) 39 (suppl 1): D70-D74 doi: 10.1093/nar/gkq1061