Element:Tntom1

Description Tntom1 is a LTR retrotransposon characterized in Nicotiana tomentosiformis (Gregor et al 2004). Tntom1 belongs to the Chromoviridae branch, term suggested (Marín and Lloréns 2000) to describe the Ty3/Gypsy elements bearing chromodomain-integrases (Malik and Eickbush 1999). The chromovirus branch is probably the most ancient phylogenetic pattern of Ty3/Gypsy retroelements (Gorinsek, Gubensek and Kordis 2004; 2005; Kordis 2005). TnTom1 is 6 Kb in size, including LTRs of 0.6 Kb. The internal region of TnTom1 displays a Primer binding site (PBS), a single ORF that codifies for a gag-pol precursor, and a Polypurine Tract (PPT) adjacent to the 3´LTR (Gregor et al 2004). This element presents a detectable chromodomain at the C-terminal end of the integrase domain. The PBS of fungi and vertebrate chromoviruses, differs significantly from that used by plant chromoviruses. While plant chromoviruses use a methionine starting tRNA (iMet), fungi and vertebrate chromoviruses use their own self-priming mechanism to start the reverse transcription (Levin 1995 ; Butler et al. 2001). Structure Figure not to scale. If present, long terminal repeats (LTRs) have been highlighted in blue. Amino acid motifs noted with lines indicate the conserved residues in each protein domain, abbreviations below mean: MA=matrix PR=protease DU or DUT=dUTPase TM=transmembrane TAV or IBMP=transactivator/viroplasmin or inclusion body matrix protein CA=capsid RT=reverse transcriptase INT=Integrase CHR=chromodomain NC=nucleocapsid RH=RNaseH SU=surface MOV=movement protein PPT=polypurine tract PBS=primer binding site ATF=aphid transmission factor VAP=virion associated protein Related literature Mobile DNA II. (Craig et al. 2002)

Genbank accession: AJ508603 Clade: Galadriel Cluster or genus: Plants Branch or class: Chromoviruses Family: Ty3/Gypsy System: LTR retroelements Host: Explore the Tree Life Project Nicotiana tomentosiformis Photo: www.knoch1.de Browse all elements