Element:Pyggy

From The Gypsy Database

Description

Pyggy is a LTR retrotransposon described in Pyrenophora graminea (Konstantinova et al. 2002). Pyggy belongs to the Chromoviridae branch, term suggested (Marín and Lloréns 2000) to describe the Ty3/Gypsy elements bearing chromodomain-integrases (Malik and Eickbush 1999). The chromovirus branch is probably the most ancient phylogenetic pattern of Ty3/Gypsy retroelements (Gorinsek, Gubensek and Kordis 2004; 2005; Kordis 2005).

To date, the available sequence of Pyggy is a degenerated clone (accession AF533704) whose genome is 6.1 Kb in size, including LTRs of 224 nt (accession AF533703). The internal region of this element displays a Primer Binding site (PBS), two pseudogenized Open Reading Frames (ORFs) for gag, and pol genes, and a Polypurine Tract (PPT) adjacent to the 3' LTR (Konstantinova et al. 2002). This element presents a detectable chromodomain at the C-terminal end of the integrase domain. The PBS of fungi and vertebrate chromoviruses, differs significantly from that used by plant chromoviruses. While plant chromoviruses use a methionine starting tRNA (iMet), fungi and vertebrate chromoviruses use their own self-priming mechanism to start the reverse transcription (Levin 1995 ; Butler et al. 2001).

Structure

Pyggy.png


Figure not to scale. If present, long terminal repeats (LTRs) have been highlighted in blue. Amino acid motifs noted with lines indicate the conserved residues in each protein domain, abbreviations below mean:

MA=matrix PR=protease DU or DUT=dUTPase TM=transmembrane TAV or IBMP=transactivator/viroplasmin or inclusion body matrix protein
CA=capsid RT=reverse transcriptase INT=Integrase CHR=chromodomain
NC=nucleocapsid RH=RNaseH SU=surface MOV=movement protein
PPT=polypurine tract PBS=primer binding site ATF=aphid transmission factor VAP=virion associated protein

Related literature

Genbank accession:AF533704
AF533703 (LTRs)
Clade:Pyggy
Cluster or genus:Fungi and vertebrates
Branch or class:Chromoviruses
Family:Ty3/Gypsy
System:LTR retroelements
Host:
Explore the Tree Life Project
Pyrenophora graminea.gif
Pyrenophora graminea
Image, courtesy of Marcel Lecomte, Skinet.be
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