GIN/GINGER TRs and INTs
RELATED FAMILIESClan AA
DOMAINSLTRs and TIRs
TREES AND NETWORKSPhylogenetic trees
Clan AA Ref. DB
LINKS OF INTEREST
From The Gypsy Database
Transposases (TRs) are often referred as the enzymes codified in DNA transposons (Class II of transposable elements) involved in double-strand DNA transposition from one location to another in the host genome ("cut and paste" mechanism).
Generically, TRs are DNA-binding enzymes that catalyze “cut and paste” or “copy and paste” reactions to promote the movement of DNA sequences (Rice and Baker 2001). TRs belong to the polynucleotidyl transferase superfamily which includes RNase H, RuvC resolvase, RAG proteins and retroviral Integrases (Nesmelova and Hackett 2010).
DDE-TRs contain a characteristic triad of conserved amino acids: Asp (D), Asp (D) and Glu (E) (this third residue can also be Asp in some cases), and a common structural motif, RNase H-like fold, bringing these three residues into close proximity to form a catalytic pocket containing two divalent metal ions that assist in the various nucleophilic reactions during DNA cleavage (Hickman et al. 2005).