Element:ScBIMV

From The Gypsy Database

Description

Sugarcane bacilliform virus (SCBV) is a pathogen of sugarcane (Saccharum Spp. and Saccharum L. interspecific hybrids) which was first observed in Cuba in 1985 (Rodriguez-Lema et al. 1985) and first purified from a sugarcane variety growing in Morocco (Lockhart and Autrey 1988). It is a plant pararetrovirus of Badnavirus genus, Caulimoviridae family (International Committee on the Taxonomy of Viruses -ICTV- Fauquet et al. 2005). To date have been completely sequenced two isolate, one from Morocco (ScBMorV, Bouhida et al. 1993) and one from Australia (ScBIMV, Geijskes et al. 2002). The SCBV sequence selected for our study is that isolated from the Australian "Ireng Maleng” variety (ScBIMV) and according to Llorens et al. 2009it is located within Class 2 of the Caulimoviridae family.

ScBIMV has non-enveloped bacilliform-shaped particles with a size of approximately 30 nm x 130 nm, although virions up to 500 nm in length have been reported (Lockhart and Autrey 1988; Geijskes et al. 2004), containing a circular dsDNA of about 7.6 Kb in size (7687 bp long) (Geijskes et al. 2002). Analysis of the genome sequence reveals that the plus strand contains three Open reading frames (ORFs I, II, III) which size, number and arrangement are similar to those of the badnaviruses (Geijskes et al. 2002). The predicted ORF I product of 21.8 kDa shows sequence similarity to the Caulimoviridae virion associated proteins (VAPs) encoded by the corresponding ORFs I. The function of ORFII product (16.4 kDa) has not yet been demonstrated, even if a DNA-binding property has been hypothesized for the ORF II products of some badnaviruses (Borah et al. 2009; Jacquot et al. 1996). The polyprotein encoded by ORF III contains the typical Badnavirus cell-to-cell movement protein (MOV), viral coat protein (COAT), aspartic protease (PR), reverse transcriptase (RT) and RNase H (RH) domains (Geijskes et al. 2002). At the C-terminus of the COAT (gag)-like domain is present the large cysteine-rich region characteristic of Badna- and Tungroviruses (Llorens et al. 2009; Hull 1996; Bouhida et al. 1993).

SCBV is present in the majority sugarcane-growing countries (Autrey et al. 1995; Comstock and Lockhart 1990) causing yield reductions in some varieties. The symptoms of infection are variable (Geijskes et al. 2002) and severely infected varieties display chlorotic leaf speckle, stunted growth, reduction in internodal elongation and premature death, while other varieties remain symptomless (Viswanathan et al. 1996; Comstock and Lockhart 1990; Lockhart and Autrey 1988). The virus is transmitted by natural vectors -as the pink (Saccharicoccus sacchari) and grey (Dysmicoccus boninsis) sugarcane mealybugs- as well as mechanically to sugarcane plants and by Agroinoculation to both rice and banana plants (Lockhart and Autrey 1988, 1991; Autrey et al. 1995; Bouhida et al. 1993). Geijskes et al. (2004) investigated the possible integration of SCBV sequences into the sugarcane genome. Their results provided evidence indicating that SCBV did not integrate into the genomes of the sugarcane cultivars tested and that the high molecular weight DNA observed were SCBV concatamers comprising up to four genome copies (derived by an open circular concatamer formation). However, considering that SCBV is hypervariable, the authors do not discard the possibility that a distant strain of SCBV is not integrated into the sugarcane genomes tested or into other sugarcane genomes.

Structure

ScBIMV.png


Figure not to scale. If present, long terminal repeats (LTRs) have been highlighted in blue. Amino acid motifs noted with lines indicate the conserved residues in each protein domain, abbreviations below mean:

MA=matrix PR=protease DU or DUT=dUTPase TM=transmembrane TAV or IBMP=transactivator/viroplasmin or inclusion body matrix protein
CA=capsid RT=reverse transcriptase INT=Integrase CHR=chromodomain
NC=nucleocapsid RH=RNaseH SU=surface MOV=movement protein
PPT=polypurine tract PBS=primer binding site ATF=aphid transmission factor VAP=virion associated protein

Related literature

Genbank accession:15029533
Clade:'
Cluster or genus:Badnavirus
Branch or class:Class 2
Family:Caulimoviridae
System:LTR retroelements
Host:
Explore the Tree Life Project
Saccharum L.jpg
Saccharum L.
Courtesy of Robert Soreng, Department of Botany, Smithsonian National Museum of Natural History,Washington D.C.
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