Element:Dane-1

From The Gypsy Database

Description

Dane-1 is a LTR retrotransposon characterized in Aspergillus nidulans (Nielsen, Hermansen and Aleksenko 2000). Dane-1 belongs to the Chromoviridae branch, term suggested (Marín and Lloréns 2000) to describe the Ty3/Gypsy elements bearing chromodomain-integrases (Malik and Eickbush 1999). The chromovirus branch is probably the most ancient phylogenetic pattern of Ty3/Gypsy retroelements (Gorinsek, Gubensek and Kordis 2004; 2005; Kordis 2005).

The genomic structure of Dane-1 is 7.7 Kb in size, including LTRs of 223 nt. The internal region of this element displays a Primer Binding Site (PBS), two Open Reading Frames (ORFs) for gag, and pol genes, and a Polypurine Tract (PPT) adjacent to the 3´LTR (Nielsen, Hermansen and Aleksenko 2000). A chromodomain is detectable at the C-terminal end of the integrase domain of this element. The PBS of fungi and vertebrate chromoviruses, differs significantly from that used by plant chromoviruses. While plant chromoviruses use a methionine starting tRNA (iMet), fungi and vertebrate chromoviruses use their own self-priming mechanism to start the reverse transcription (Levin 1995 ; Butler et al. 2001).

Structure

Dane-1.png


Figure not to scale. If present, long terminal repeats (LTRs) have been highlighted in blue. Amino acid motifs noted with lines indicate the conserved residues in each protein domain, abbreviations below mean:

MA=matrix PR=protease DU or DUT=dUTPase TM=transmembrane TAV or IBMP=transactivator/viroplasmin or inclusion body matrix protein
CA=capsid RT=reverse transcriptase INT=Integrase CHR=chromodomain
NC=nucleocapsid RH=RNaseH SU=surface MOV=movement protein
PPT=polypurine tract PBS=primer binding site ATF=aphid transmission factor VAP=virion associated protein

Related literature

Genbank accession:AF295689
Clade:Maggy
Cluster or genus:Fungi and vertebrates
Branch or class:Chromoviruses
Family:Ty3/Gypsy
System:LTR retroelements
Host:
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Aspergillus nidulans.gif
Aspergillus nidulans
Image, courtesy of Ralph Cavaliere, Gettysburg College
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